8/17/2023 0 Comments Define mogWe classified a T-cell response as positive if proliferation (measured by a cell division index ≥3) was confirmed by the secretion of at least one cytokine. Additionally, human leukocyte antigen (HLA)-DQ and HLA-DR genotyping of all participants was performed. Antigen-specific T-cell responses were assessed using the carboxyfluorescein diacetate succinimidyl ester proliferation assay and the detection of granulocyte macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-ɤ and interleukin (IL)-4, IL-6, and IL-17A in cell culture supernatants. Peripheral blood mononuclear cell cultures were stimulated with eight AQP4 and nine MOG peptides selected from previous studies and a tetanus toxoid peptide mix as a positive control. Therefore, we compared AQP4 and MOG specific peripheral T-cell response in individuals with AQP4-Ab ( n = 8), MOG-Ab ( n = 10), multiple sclerosis (MS, n = 8), and healthy controls (HC, n = 14). However, no study has yet analyzed the presence of MOG reactive T-cells in patients with MOG antibodies. Previous studies have shown that not only antibodies, but also autoreactive T-cell responses against AQP4 are present in NMOSD. 5Department of Neurology, Medical University of Vienna, Vienna, AustriaĪutoantibodies against aquaporin-4 (AQP4-Ab) and myelin oligodendrocyte glycoprotein (MOG-Ab) are associated with rare central nervous system inflammatory demyelinating diseases like neuromyelitis optica spectrum disorders (NMOSD).4Department of Neuroimmunology, University of Zurich, Zurich, Switzerland.3Paediatric Neurology, Children's Hospital Datteln, Witten/Herdecke University, Datteln, Germany.2Oxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom.
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